Understanding the importance of the force associated to the domain movements of BiP during protein translocation in the endoplasmic reticulum. To determine the mechano-chemistry of protein transport, we will use optical tweezers (OT) to characterize the dynamics and force generating step(s) during the polypeptide translocation mediated by Bip and SecA at the single molecule level.
Team
- Christian A.M. Wilson Moya (Uchile, Biochemistry)
- Diego Quiroga (Uchile, Biochemistry)
- Francesca Burgos (Uchile, Biochemistry)
- Hilda Alfaro (Uchile, Biochemistry)
- Miguel Lagos (Uchile, Biochemistry)
Article 2018
Book chapter 2018
Mechanical Properties of Chaperone BiP, the Master Regulator of the Endoplasmic Reticulum
Review 2022: Determination of protein–protein interactions at the single-molecule level using optical tweezers
Review 2024:Towards the understanding of molecular motors and its relationship with local unfolding
Article 2024: Characterization of the interaction between the Sec61 translocon complex and ppαF using optical tweezers
